We were able to immobilize IgG molecules in an oriented form onto the protein-A attached surfaces, especially in the Of EDA molecules on the substrate surface, especially onto the aggregates by using a carbodiimide (i.e., EDAC) as the activatingĪgent. Protein-A molecules were bound to the free amino groups

Microscopy (AFM) images exhibited aggregate formation at this step. On the surfaces, while EDA deposition increased with the dipping time significantly. There was no effect of concentration on the formation of EDA molecules 2,2′-(ethylenedioxy)diethylamine (EDA) molecules were then covalentlyĪttached to the silicon surface with GPTS molecules. Dipping time and GPTS concentration were found to be effectedīy on both layer thicknesses and water-contact angles. (GPTS) molecules were self-assembled onto these substrates. After hydroxylation of the Si(001) surfaces, 3-glycidoxypropyltrimethoxysilane Was applied for oriented immobilization of IgG in this study. The aim of this study was the oriented immobilization of IgG molecules on the silicon surfaces.

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